2IVK
Crystal structure of the periplasmic endonuclease Vvn complexed with a 16-bp DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Collection date | 2005-12-17 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 64.636, 64.705, 79.157 |
| Unit cell angles | 74.45, 73.56, 75.56 |
Refinement procedure
| Resolution | 37.090 - 2.900 |
| R-factor | 0.237 |
| Rwork | 0.237 |
| R-free | 0.28400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oup |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.090 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.080 | 0.300 |
| Number of reflections | 24597 | |
| <I/σ(I)> | 17.5 | 3.3 |
| Completeness [%] | 97.2 | 97.2 |
| Redundancy | 3.6 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 20% PEG3350, 0.2 M DI-SODIUM TARTRATE DEHYDRATE, pH 7.00 |
