2IU6

REGULATION OF THE DHA OPERON OF LACTOCOCCUS LACTIS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	110
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS-IV
Spacegroup name	P 32 2 1
Unit cell lengths	101.090, 101.090, 148.600
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.000
R-factor	0.173
Rwork	0.173
R-free	0.19800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1oi2
RMSD bond length	0.013
RMSD bond angle	1.662
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.040
High resolution limit [Å]	2.000	2.000
Rmerge	0.080	0.450
Number of reflections	59910
<I/σ(I)>	22.6	6.88
Completeness [%]	100.0	100
Redundancy	12.38	10.95

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7		0.15 M DL-MALIC ACID PH 7.0, 20% PEG-3350