2ITO
Crystal structure of EGFR kinase domain G719S mutation in complex with Iressa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-10-24 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | I 2 3 |
Unit cell lengths | 142.929, 142.929, 142.929 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.510 - 3.250 |
R-factor | 0.197 |
Rwork | 0.189 |
R-free | 0.26600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1m17 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.686 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.500 |
High resolution limit [Å] | 3.250 | 3.250 |
Rmerge | 0.090 | 0.390 |
Number of reflections | 8145 | |
<I/σ(I)> | 29.2 | 5.9 |
Completeness [%] | 99.9 | 100 |
Redundancy | 7.1 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 1.2M KNA TARTRATE, 0.1M HEPES 7.5 |