2ISY
Crystal structure of the nickel-activated two-domain iron-dependent regulator (IdeR)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-12-14 |
| Wavelength(s) | 1.03320 |
| Spacegroup name | P 1 |
| Unit cell lengths | 41.954, 44.743, 49.641 |
| Unit cell angles | 110.18, 96.57, 108.29 |
Refinement procedure
| Resolution | 45.130 - 1.955 |
| R-factor | 0.178 |
| Rwork | 0.176 |
| R-free | 0.21100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fx7 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.282 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 4.200 | 1.950 |
| Rmerge | 0.060 | 0.045 | 0.173 |
| Number of reflections | 20610 | ||
| <I/σ(I)> | 12.6 | ||
| Completeness [%] | 91.8 | 94.7 | 58.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.2 | 298 | 0.6 M Na/K phosphate pH 8.2, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
