2IRY
Crystal Structure of the Polymerase Domain from Mycobacterium tuberculosis Ligase D with dGTP and Manganese.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-07-16 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54180 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.221, 75.870, 95.809 |
| Unit cell angles | 90.00, 92.59, 90.00 |
Refinement procedure
| Resolution | 40.490 - 1.780 |
| R-factor | 0.18453 |
| Rwork | 0.182 |
| R-free | 0.23034 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.414 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.490 | 1.880 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Rmerge | 0.070 | 0.175 |
| Number of reflections | 56549 | |
| <I/σ(I)> | 15.5 | 4.3 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 285 | 0.1M Na Hepes, 0.01M MnCl2, 10%(v/v) iso-propanol, 20% PEG 4000, 0.01M dGTP, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 285K |
