2IRV
Crystal structure of GlpG, a rhomboid intramembrane serine protease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-05-01 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.541 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.820, 69.350, 67.580 |
| Unit cell angles | 90.00, 101.03, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.300 |
| Rwork | 0.220 |
| R-free | 0.26300 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.301 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 17028 | |
| <I/σ(I)> | 9.8 | 1.8 |
| Completeness [%] | 98.4 | 97.2 |
| Redundancy | 3 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 100 mM MES, 30% PEG 400, 200 mM CaCl2 in reservoir, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
