2IQ9
PFA2 FAB fragment, triclinic apo form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 113 |
Detector technology | IMAGE PLATE |
Collection date | 2005-10-12 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 |
Unit cell lengths | 41.564, 42.776, 58.355 |
Unit cell angles | 95.81, 94.81, 91.11 |
Refinement procedure
Resolution | 20.700 - 2.300 |
R-factor | 0.215 |
Rwork | 0.211 |
R-free | 0.27800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ncw |
RMSD bond length | 0.010 |
RMSD bond angle | 1.332 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 22.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 16504 | |
<I/σ(I)> | 10.8 | 2 |
Completeness [%] | 93.3 | 92.8 |
Redundancy | 1.7 | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.5 | 294 | 25% PEG3350, 0.1 M Tris, pH 8.5, VAPOR DIFFUSION, temperature 294K |