2IQ6

Crystal Structure of the Aminopeptidase from Vibrio proteolyticus in Complexation with Leucyl-leucyl-leucine.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2005-05-04
Detector	RIGAKU RAXIS IV
Spacegroup name	P 61 2 2
Unit cell lengths	108.400, 108.400, 96.800
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.000
R-factor	0.206
Rwork	0.206
R-free	0.24700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1cp6
RMSD bond length	0.005
RMSD bond angle	1.500
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.000	2.000
Number of reflections	23236
<I/σ(I)>	12.3	4
Completeness [%]	96.2	78.3
Redundancy	22.6	21.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	KSCN, NACL, TRIS, PH 8.0, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 293K, TRIS REPLACED BY HEPES