2IPZ
A Parallel Coiled-Coil Tetramer with Offset Helices
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-10-07 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 23.783, 53.878, 52.016 |
Unit cell angles | 90.00, 95.77, 90.00 |
Refinement procedure
Resolution | 51.990 - 1.350 |
R-factor | 0.20576 |
Rwork | 0.202 |
R-free | 0.23709 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2b22 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.260 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.000 | 1.370 |
High resolution limit [Å] | 1.350 | 1.350 |
Rmerge | 0.047 | 0.395 |
Number of reflections | 27103 | |
<I/σ(I)> | 17.5 | 4.9 |
Completeness [%] | 94.4 | 95.3 |
Redundancy | 3.4 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 295 | 10% Iso-propanol, 20% PEG 4000, 0.1M sodium bromide, 0.1M sodium HEPES, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K |