2IPX
Human Fibrillarin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-10-05 |
| Detector | RIGAKU |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 57.261, 57.261, 135.028 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.000 - 1.820 |
| R-factor | 0.20241 |
| Rwork | 0.200 |
| R-free | 0.24552 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fbn |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.402 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.095 | 0.574 |
| Number of reflections | 20921 | |
| <I/σ(I)> | 11.3 | 1.6 |
| Completeness [%] | 96.3 | 65.4 |
| Redundancy | 11.9 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 300 | 20% PEG3350, 0.2 M CaCl2, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
