2IPU
PFA1 Fab fragment complexed with Abeta 1-8 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-04-20 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 |
| Unit cell lengths | 43.070, 70.335, 74.197 |
| Unit cell angles | 72.32, 86.08, 86.02 |
Refinement procedure
| Resolution | 23.530 - 1.650 |
| R-factor | 0.184 |
| Rwork | 0.182 |
| R-free | 0.22500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2iq9 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.436 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.530 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Number of reflections | 90408 | |
| <I/σ(I)> | 2.4 | |
| Completeness [%] | 90.7 | 66.3 |
| Redundancy | 3.1 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 294 | 0.1 M Tris, 25% PEG3350, pH 8.5, VAPOR DIFFUSION, temperature 294K |
