2IPT
PFA1 Fab Fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-03-27 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 |
| Unit cell lengths | 41.985, 42.825, 58.381 |
| Unit cell angles | 95.84, 94.77, 91.91 |
Refinement procedure
| Resolution | 25.190 - 2.000 |
| R-factor | 0.18 |
| Rwork | 0.177 |
| R-free | 0.22300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2iq9 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.429 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.190 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 25317 | |
| Completeness [%] | 93.1 | 85.1 |
| Redundancy | 1.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 294 | 0.1 M Hepes, 25% PEG3350, pH 7.5, VAPOR DIFFUSION, temperature 294K |
