2IP2
Structure of the Pyocyanin Biosynthetic Protein PhzM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-08-04 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.541 |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.970, 62.410, 68.750 |
| Unit cell angles | 97.47, 105.37, 108.09 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| R-factor | 0.1963 |
| Rwork | 0.194 |
| R-free | 0.24739 |
| Structure solution method | SAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.744 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | RESOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.400 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.110 | 0.371 |
| Number of reflections | 60747 | |
| <I/σ(I)> | 11.3 | 2.6 |
| Completeness [%] | 94.2 | 90.7 |
| Redundancy | 7.36 | 7.31 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | The well solution contained 18-21% PEG 3350, 0.1M magnesium chloride, 0.1M MOPS, pH 7.0. The drops were formed by mixing equal volumes of protein (13.5 mg/ml) and well solution, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
