2IOQ

Crystal Structure of full-length HTPG, the Escherichia coli HSP90

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2003-08-11
Detector	ADSC QUANTUM 315
Wavelength(s)	1.08
Spacegroup name	P 65 2 2
Unit cell lengths	105.124, 105.124, 531.077
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	58.700 - 3.500
Rwork	0.329
R-free	0.37000
Structure solution method	MIR
RMSD bond length	0.002
RMSD bond angle	0.684
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.700	3.630
High resolution limit [Å]	3.500	3.500
Number of reflections	22816
<I/σ(I)>	21.4	2.6
Completeness [%]	98.4	98.2
Redundancy	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	5-10% PEG3000 or PEG4000, 30-60 mM MgCl2, 50 mM Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K