2IOP

Crystal Structure of Full-length HtpG, the Escherichia coli Hsp90, Bound to ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2004-08-19
Detector	ADSC QUANTUM 315
Wavelength(s)	1.08
Spacegroup name	P 1 21 1
Unit cell lengths	78.618, 145.060, 165.352
Unit cell angles	90.00, 92.43, 90.00

Refinement procedure

Resolution	46.600 - 3.550
Rwork	0.317
R-free	0.35700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.741
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.600	3.630
High resolution limit [Å]	3.550	3.550
Number of reflections	36251
<I/σ(I)>	11.8	2.3
Completeness [%]	78.5	32.4
Redundancy	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1-2 M Ammonium Sulfate, 0.5 M LiCl, VAPOR DIFFUSION, HANGING DROP, temperature 293K