2IOO

Crystal structure of the mouse p53 core domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	92.15
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	91.568, 44.709, 62.607
Unit cell angles	90.00, 125.61, 90.00

Refinement procedure

Resolution	50.000 - 2.020
Rwork	0.210
R-free	0.22500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Mouse p53 core domain
RMSD bond length	0.006
RMSD bond angle	1.330
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	50.000
High resolution limit [Å]	2.020	2.020
Rmerge	0.044	0.198
Number of reflections	13086
<I/σ(I)>	5.82	5.82

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	100mM Hepes, 16-18% PEG 2K MME, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K