2INY
Nanoporous Crystals of Chicken Embryo Lethal Orphan (CELO) Adenovirus Major Coat Protein, Hexon
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 157.770, 157.770, 114.200 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.060 - 3.900 |
| Rwork | 0.372 |
| R-free | 0.41600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1p30 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.970 |
| High resolution limit [Å] | 3.900 | 3.900 |
| Number of reflections | 14636 | |
| <I/σ(I)> | 7.1 | 2.5 |
| Completeness [%] | 95.7 | 94.4 |
| Redundancy | 4.6 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 7% iso-propanol, 13% PEG 4000, 0.01 M glycine, 0.1 M Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K |
