2INF
Crystal Structure of Uroporphyrinogen Decarboxylase from Bacillus subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BSRF BEAMLINE 3W1A |
| Synchrotron site | BSRF |
| Beamline | 3W1A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-12-06 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 58.612, 80.410, 90.940 |
| Unit cell angles | 68.68, 89.64, 80.82 |
Refinement procedure
| Resolution | 30.000 - 2.300 |
| R-factor | 0.20013 |
| Rwork | 0.197 |
| R-free | 0.25125 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uro |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.290 |
| Data reduction software | AUTOMAR |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 65152 | |
| Completeness [%] | 96.2 | 96.2 |
| Redundancy | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 25% PEG 2000, 100mM citrate, 160mM sodium acetate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
