2IMW
Mechanism of Template-Independent Nucleotide Incorporation Catalyzed by a Template-Dependent DNA Polymerase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 173 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-07-31 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 98.057, 101.857, 52.380 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.730 - 2.050 |
| R-factor | 0.232 |
| Rwork | 0.230 |
| R-free | 0.28400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | : 1JX4 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.303 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.090 |
| High resolution limit [Å] | 2.050 | 5.550 | 2.050 |
| Rmerge | 0.063 | 0.044 | 0.340 |
| Number of reflections | 32881 | ||
| <I/σ(I)> | 16.7 | ||
| Completeness [%] | 97.5 | 79.2 | 93.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 10% PEG3350, 0.1 M Calcium acetate, 0.1 M hepes pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG3350 | ||
| 2 | 1 | 1 | Calcium acetate | ||
| 3 | 1 | 1 | hepes | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | PEG3350 | ||
| 6 | 1 | 2 | Calcium acetate | ||
| 7 | 1 | 2 | hepes |
