2IM0

Crystal structure of poliovirus polymerase complexed with CTP and Mg2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Detector	NOIR-1
Wavelength(s)	0.98
Spacegroup name	P 65
Unit cell lengths	127.695, 127.695, 113.034
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.800 - 2.250
Rwork	0.237
R-free	0.26000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ra6
RMSD bond length	0.007
RMSD bond angle	1.400
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.330
High resolution limit [Å]	2.250	2.250
Number of reflections	49540
<I/σ(I)>	3.6	3.6
Completeness [%]	99.7	99.5
Redundancy	7.53	6.82

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	289	sodium acetate, cacodylate, dtt, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K