2ILV
crystal structure of multifunctional sialyltransferase from Pasteurella multocida with CMP and alpha-lactose bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97945 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.912, 63.627, 63.782 |
| Unit cell angles | 90.00, 98.36, 90.00 |
Refinement procedure
| Resolution | 63.120 - 2.270 |
| R-factor | 0.21911 |
| Rwork | 0.217 |
| R-free | 0.25725 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ex1 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.402 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.630 | 2.210 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Number of reflections | 25036 | |
| <I/σ(I)> | 9.6 | 2.1 |
| Completeness [%] | 93.5 | 95.3 |
| Redundancy | 2.3 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 30%, PEG MME 2000, 0.2 M NaBr, 0.4% Triton X-100 soaked in 20 mM alpha-lactose for one hour, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
