2IJG

Crystal Structure of cryptochrome 3 from Arabidopsis thaliana

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2006-06-10
Detector	ADSC QUANTUM 1
Wavelength(s)	0.97926
Spacegroup name	P 61 2 2
Unit cell lengths	117.897, 117.897, 216.567
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.100
Rwork	0.238
R-free	0.26190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1np7
RMSD bond length	0.006
RMSD bond angle	1.490
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.100
Rmerge	0.200
Number of reflections	52546
<I/σ(I)>	14.6
Completeness [%]	100.0
Redundancy	10.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.4	294	pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 294K