2IJ0
Structural basis of T cell specificity and activation by the bacterial superantigen toxic shock syndrome toxin-1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F2 |
Synchrotron site | CHESS |
Beamline | F2 |
Wavelength(s) | 0.978 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 190.898, 68.372, 53.549 |
Unit cell angles | 90.00, 106.24, 90.00 |
Refinement procedure
Resolution | 25.000 - 2.250 |
Rwork | 0.242 |
R-free | 0.25200 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | CNS |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 25.000 |
High resolution limit [Å] | 2.250 |
Number of reflections | 32180 |
<I/σ(I)> | 21.5 |
Completeness [%] | 99.4 |
Redundancy | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.1M Sodium Cacodylate, 0.2 M Magnesium Acetate, PEG 8000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |