2IIR
Acetate kinase from a hypothermophile Thermotoga maritima
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Detector technology | CCD |
| Collection date | 2004-10-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.90000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 105.434, 300.333, 334.898 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.300 |
| R-factor | 0.23 |
| Rwork | 0.229 |
| R-free | 0.25300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.277 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 3.420 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.090 | 0.264 |
| Number of reflections | 78832 | |
| <I/σ(I)> | 10.5 | |
| Completeness [%] | 97.9 | 88.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 293 | 100mM Ches, 36% MPD, 50mM Calcium Chloride, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
