2IIP
Human Nicotinamide N-methyltransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-09-20 |
| Detector | RIGAKU |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.016, 62.007, 107.073 |
| Unit cell angles | 82.35, 81.89, 68.30 |
Refinement procedure
| Resolution | 29.500 - 2.050 |
| R-factor | 0.21903 |
| Rwork | 0.217 |
| R-free | 0.26477 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.785 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.500 | 2.120 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.091 | 0.578 |
| Number of reflections | 64271 | |
| <I/σ(I)> | 7.7 | 1.4 |
| Completeness [%] | 97.6 | 93.9 |
| Redundancy | 2.2 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 300 | PEG, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
