2IIF
single chain Integration Host Factor mutant protein (scIHF2-K45aE) in complex with DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 103 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-10-01 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.542 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.239, 54.444, 177.865 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.720 |
| R-factor | 0.238 |
| Rwork | 0.237 |
| R-free | 0.27400 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.850 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.870 |
| High resolution limit [Å] | 2.720 | 2.720 |
| Number of reflections | 12530 | |
| Completeness [%] | 97.1 | 95.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 1:1 mix water/ well solution containing: 50mM Tris(pH 7.5), 25% PEG5000-MME, 20% glycerol, 50mM NaCl, 7.5mM MnCl2, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG5000-MME | ||
| 10 | 1 | 2 | H2O | ||
| 2 | 1 | 1 | Tris | ||
| 3 | 1 | 1 | glycerol | ||
| 4 | 1 | 1 | sodium chloride | ||
| 5 | 1 | 1 | magnesium chloride | ||
| 6 | 1 | 1 | H2O | ||
| 7 | 1 | 2 | PEG5000-MME | ||
| 8 | 1 | 2 | sodium chloride | ||
| 9 | 1 | 2 | magnesium chloride |
