2IIA
Anabaena sensory rhodopsin transducer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-09-03 |
| Spacegroup name | P 4 |
| Unit cell lengths | 57.274, 57.274, 39.788 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.620 - 1.800 |
| R-factor | 0.203 |
| Rwork | 0.202 |
| R-free | 0.23800 |
| Structure solution method | SAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.731 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 4.880 | 1.800 |
| Rmerge | 0.031 | 0.018 | 0.214 |
| Number of reflections | 11936 | ||
| <I/σ(I)> | 15.9 | ||
| Completeness [%] | 98.5 | 86.8 | 92 |
| Redundancy | 4.5 | 4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 100 mM sodium acetate, pH 4.6 and 7 to 11% (w/v) polyethylene glycol (PEG) 8000, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
