2II0
Crystal Structure of catalytic domain of Son of sevenless (Rem-Cdc25) in the absence of Ras
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-09-15 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 82.771, 66.799, 119.653 |
Unit cell angles | 90.00, 108.91, 90.00 |
Refinement procedure
Resolution | 34.390 - 2.020 |
R-factor | 0.218 |
Rwork | 0.218 |
R-free | 0.24800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | pdb model 1NVV (Rem and Cdc25 domain separately) |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.090 |
High resolution limit [Å] | 2.020 | 2.020 |
Number of reflections | 39181 | |
<I/σ(I)> | 16.5 | 2.3 |
Completeness [%] | 95.5 | 76.8 |
Redundancy | 5.9 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 20% PEG3350, 0.2M ammonium chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |