2IHN
Co-crystal of Bacteriophage T4 RNase H with a fork DNA substrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-01-21 |
| Detector | RIGAKU SATURN 92 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.160, 84.990, 89.290 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.370 - 3.000 |
| R-factor | 0.225 |
| Rwork | 0.222 |
| R-free | 0.28200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tfr |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.369 |
| Data reduction software | CrystalClear |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.370 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.091 | 0.328 |
| Number of reflections | 10803 | |
| <I/σ(I)> | 12.7 | 3.9 |
| Completeness [%] | 99.2 | 99.8 |
| Redundancy | 3.81 | 3.74 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 295 | 18.5% (w/v) PEG 2000 Monomethylether, 0.1M Tris-HCl, 20% (w/v) glucose, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 2000 Monomethylether | ||
| 2 | 1 | 1 | Tris-HCl | ||
| 3 | 1 | 1 | glucose | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | PEG 2000 Monomethylether | ||
| 6 | 1 | 2 | Tris-HCl | ||
| 7 | 1 | 2 | glucose | ||
| 8 | 1 | 2 | H2O |
