2IGO
Crystal structure of pyranose 2-oxidase H167A mutant with 2-fluoro-2-deoxy-D-glucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-22 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.092 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 168.590, 103.080, 169.040 |
| Unit cell angles | 90.00, 106.30, 90.00 |
Refinement procedure
| Resolution | 38.900 - 1.950 |
| R-factor | 0.184 |
| Rwork | 0.184 |
| R-free | 0.22064 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.907 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 38.900 |
| High resolution limit [Å] | 1.950 |
| Number of reflections | 403903 |
| <I/σ(I)> | 14.2 |
| Completeness [%] | 99.8 |
| Redundancy | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 298 | 12-13% monomethyl ether PEG 2000, 0.1 M mes, 50 mM CaCl2, 25% glycerol , pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
