2IGK
Crystal structure of recombinant pyranose 2-oxidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-09-22 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.095 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 168.780, 103.860, 169.260 |
| Unit cell angles | 90.00, 106.36, 90.00 |
Refinement procedure
| Resolution | 39.200 - 1.800 |
| R-factor | 0.17692 |
| Rwork | 0.177 |
| R-free | 0.21421 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.767 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 39.200 |
| High resolution limit [Å] | 1.700 |
| Number of reflections | 517862 |
| <I/σ(I)> | 12.2 |
| Completeness [%] | 99.0 |
| Redundancy | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 298 | 12-13% monomethyl ether PEG 2000, 0.1 M mes, 50 mM CaCl2, 25% glycerol, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
