2IF5
Structure of the POZ domain of human LRF, a master regulator of oncogenesis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 65.850, 65.850, 162.603 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.080 - 2.000 |
| R-factor | 0.23245 |
| Rwork | 0.229 |
| R-free | 0.29096 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1buo |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.580 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.130 |
| High resolution limit [Å] | 2.000 | 2.030 |
| Rmerge | 0.096 | 0.259 |
| Number of reflections | 12540 | |
| <I/σ(I)> | 25.9 | 9 |
| Completeness [%] | 98.2 | 99.8 |
| Redundancy | 13 | 13 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 290 | Protein at 24 mg/ml in 25 mM Tris, 150 mM NaCl, 2mM DTT, 0.18 M Calcium acetate hydrate, 18% w/v Polyethylene glycol 3350, 0.01 M Praseodymium (III) Acetate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
