2IEZ
Crystal Structure of mouse Rab27b bound to GDP in monoclinic space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 170.496, 63.348, 80.845 |
| Unit cell angles | 90.00, 98.45, 90.00 |
Refinement procedure
| Resolution | 35.600 - 2.800 |
| R-factor | 0.26743 |
| Rwork | 0.262 |
| R-free | 0.36315 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2iey |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.189 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 84.210 | 2.800 |
| High resolution limit [Å] | 2.710 | 2.700 |
| Rmerge | 0.121 | 0.484 |
| Number of reflections | 20069 | |
| Completeness [%] | 86.2 | 76.4 |
| Redundancy | 3 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 289 | MPD, CaCl2, Hepes, pH 7.0, VAPOR DIFFUSION, temperature 289K |
