2IEQ
Core Structure of S2 from the Human Coronavirus NL63 Spike Glycoprotein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-04-04 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.020, 51.688, 54.080 |
| Unit cell angles | 90.00, 107.56, 90.00 |
Refinement procedure
| Resolution | 51.570 - 1.747 |
| R-factor | 0.19866 |
| Rwork | 0.196 |
| R-free | 0.23977 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zv8 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.502 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.570 | 1.810 |
| High resolution limit [Å] | 1.747 | 1.747 |
| Rmerge | 0.068 | 0.363 |
| Number of reflections | 25668 | |
| <I/σ(I)> | 10.7 | 3.7 |
| Completeness [%] | 95.6 | 98.1 |
| Redundancy | 3.2 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 298 | PEG 400, sodium acetate, Imidazole, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
