2IEG

Crystal structure of rabbit muscle glycogen phosphorylase in complex with 3,4-dihydro-2-quinolone

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	100
Detector technology	CCD
Collection date	2002-02-01
Detector	ADSC QUANTUM 4
Spacegroup name	P 21 21 21
Unit cell lengths	114.120, 125.156, 128.387
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.970 - 1.900
R-factor	0.23028
Rwork	0.228
R-free	0.26931
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.486
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	AMoRE
Refinement software	REFMAC (5.1.17)

Data quality characteristics

	Overall
Low resolution limit [Å]	32.970
High resolution limit [Å]	1.900
Number of reflections	111798
Completeness [%]	81.3
Redundancy	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.7	298	10mM BES, pH6.7, 0.1mM EDTA, VAPOR DIFFUSION, temperature 298K