2IE1
Polyamines stabilize left-handed Z-DNA. We found new type of polyamine which stabilize left-handed Z-DNA by X-ray crystallography
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2005-06-28 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 17.640, 30.380, 43.630 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.600 |
| R-factor | 0.202 |
| Rwork | 0.190 |
| R-free | 0.20500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dj6 in PDB |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MERLOT |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.055 | 0.055 |
| Number of reflections | 3249 | |
| <I/σ(I)> | 6400 | 6400 |
| Completeness [%] | 97.1 | 97.1 |
| Redundancy | 4.8 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
