2ICU
Crystal Structure of Hypothetical Protein YedK From Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-24 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97240 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.687, 67.366, 75.125 |
| Unit cell angles | 90.00, 98.29, 90.00 |
Refinement procedure
| Resolution | 18.590 - 1.600 |
| R-factor | 0.17417 |
| Rwork | 0.173 |
| R-free | 0.20319 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SCA2STRUCTURE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 53997 | |
| <I/σ(I)> | 27.09 | 4.5 |
| Completeness [%] | 94.1 | 67 |
| Redundancy | 6.8 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.4 | 291 | 0.5 MICROLITER DROPS CONTAINING EQUAL VOLUMES OF PROTEIN CONCENTRATE (9.9 MG/ML) AND RESERVOIR SOLUTION CONTAINING 50% PEG 3350, 0.1M BIS-TRIS PH 6.4, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
