2ICQ
urate oxidase under 2.0 MPa pressure of nitrous oxide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE DW32 |
| Synchrotron site | LURE |
| Beamline | DW32 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-12-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.964 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 80.189, 96.034, 105.212 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.770 - 1.750 |
| R-factor | 0.16642 |
| Rwork | 0.165 |
| R-free | 0.18779 |
| Structure solution method | RIGID BODY |
| Starting model (for MR) | 2iba |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.476 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.055 | 0.346 |
| Number of reflections | 41239 | |
| <I/σ(I)> | 13.3 | |
| Completeness [%] | 99.9 | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 10.5 | 298 | urate oxidase 8.5mg/ml, sodium carbonate 10mM, 8-azaxanthin 0.2mg/ml, PEG 8000 7% , pH 10.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
