2IC0
Urate oxidase under 2.0 MPa pressure of xenon
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE DW32 |
| Synchrotron site | LURE |
| Beamline | DW32 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-03-15 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.964 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 80.502, 96.583, 106.005 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.840 - 1.780 |
| R-factor | 0.166 |
| Rwork | 0.164 |
| R-free | 0.20100 |
| Structure solution method | rigid body |
| Starting model (for MR) | 2iba |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.501 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.049 | 0.049 |
| Number of reflections | 34588 | |
| Completeness [%] | 82.5 | 44.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 4MG/ML URATE OXIDASE, 8-AZAXANTHINE 0.2MG/ML, TRIS 50MM, PEG 8000 2-5%, NACL 100MM, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 298K |
