2IBA
Urate oxidase from Aspergillus flavus complexed with its inhibitor 8-azaxanthine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 277 |
| Detector technology | CCD |
| Collection date | 2005-11-04 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.972 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 80.492, 96.041, 105.343 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.870 - 1.500 |
| R-factor | 0.177 |
| Rwork | 0.176 |
| R-free | 0.19700 |
| Structure solution method | RIGID BODY |
| Starting model (for MR) | 1r51 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.408 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.056 | 0.271 |
| Number of reflections | 59653 | |
| <I/σ(I)> | 10.8 | |
| Completeness [%] | 91.1 | 87.9 |
| Redundancy | 5.4 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 298 | 10MG/ML URATE OXIDASE, 8-AZAXANTHINE 0.2MG/ML, TRIS 20mM, PEG 8000 7%, NACL 200mM, batch, TEMPERATURE 298K, PH 8.5 |
