2IB5
Structural characterization of a blue chromoprotein and its yellow mutant from the sea anemone cnidopus japonicus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-12-09 |
| Detector | SBC-2 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.860, 126.850, 100.510 |
| Unit cell angles | 90.00, 102.10, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.800 |
| R-factor | 0.195 |
| Rwork | 0.195 |
| R-free | 0.22500 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.673 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.077 | |
| Number of reflections | 335346 | |
| <I/σ(I)> | 12 | 5.1 |
| Completeness [%] | 99.3 | 92 |
| Redundancy | 52.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 0.2M NaH2PO4, 20mM Tris-HCl, 150mM NaCl, 20% PEG3350, 20% glycerol, 2mM TCEP, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
