2IB0

Crystal structure of a conserved hypothetical protein, rv2844, from Mycobacterium tuberculosis

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2006-08-23
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9795,0.9800,0.96
Spacegroup name	P 41 21 2
Unit cell lengths	98.029, 98.029, 91.337
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.850 - 2.000
R-factor	0.204
Rwork	0.202
R-free	0.23500
Structure solution method	MAD
Starting model (for MR)	None
RMSD bond length	0.018
RMSD bond angle	1.575
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.054	0.345
Number of reflections	26492
<I/σ(I)>	13.2	2
Completeness [%]	85.8	38.7
Redundancy	6	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	LiSO4 0.2M Cacodylate pH7 0.1M PEG400 32%, VAPOR DIFFUSION, SITTING DROP, temperature 298K
1	VAPOR DIFFUSION, SITTING DROP	7	298	LiSO4 0.2M Cacodylate pH7 0.1M PEG400 32%, VAPOR DIFFUSION, SITTING DROP, temperature 298K