2IAN

Structural basis for recognition of mutant self by a tumor-specific, MHC class II-restricted TCR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2005-08-25
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.54
Spacegroup name	P 1 21 1
Unit cell lengths	93.474, 242.866, 105.222
Unit cell angles	90.00, 111.32, 90.00

Refinement procedure

Resolution	45.450 - 2.800
R-factor	0.22496
Rwork	0.221
R-free	0.29590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fyt 1ial
RMSD bond length	0.013
RMSD bond angle	1.532
Phasing software	PHASER
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.940
High resolution limit [Å]	2.800	2.800
Rmerge	0.082	0.235
Number of reflections	100306
<I/σ(I)>	11.1	3.9
Completeness [%]	93.8	92.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	295	10% PEG6000, 0.1M di-ammonium phosphate, 0.1M Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K