2IAI
Crystal structure of SCO3833, a member of the TetR transcriptional regulator family from Streptomyces coelicolor A3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 2006-06-09 |
| Detector | SBC-3 |
| Wavelength(s) | 0.97940 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 61.308, 61.308, 113.865 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 53.070 - 1.650 |
| R-factor | 0.17042 |
| Rwork | 0.169 |
| R-free | 0.20820 |
| Structure solution method | SAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.654 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.070 | 1.693 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.062 | 0.388 |
| Number of reflections | 30460 | |
| <I/σ(I)> | 9.2 | 3.7 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 10.9 | 11.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 293 | Crystallized from mother liquor containing 0.1 M Tris-HCl pH=8.5 and 7%(w/v) PEG 8000, VAPOR DIFFUSION, temperature 293K |
