2IAE
Crystal structure of a protein phosphatase 2A (PP2A) holoenzyme.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-03 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 265.301, 265.301, 265.301 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.500 |
| R-factor | 0.2598 |
| Rwork | 0.257 |
| R-free | 0.31552 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.380 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.630 |
| High resolution limit [Å] | 3.500 | 7.530 | 3.500 |
| Rmerge | 0.118 | 0.046 | 0.905 |
| Number of reflections | 76332 | ||
| <I/σ(I)> | 7.5 | ||
| Completeness [%] | 97.5 | 93 | 98.1 |
| Redundancy | 4.4 | 4.4 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 0.1M MES, pH 7.0, 50mM NaCl, 1.4M ammonium sulfate, 0.2M LiCl, 2% 1,6-diaminohexane, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
