2I8E
Structure of SSO1404, a predicted DNA repair-associated protein from Sulfolobus solfataricus P2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 2006-06-09 |
| Detector | SBC-3 |
| Wavelength(s) | 0.97940 |
| Spacegroup name | P 62 |
| Unit cell lengths | 64.277, 64.277, 39.529 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.940 - 1.590 |
| R-factor | 0.18972 |
| Rwork | 0.188 |
| R-free | 0.22708 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.692 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.650 |
| High resolution limit [Å] | 1.590 | 1.590 |
| Rmerge | 0.052 | 0.309 |
| Number of reflections | 12619 | |
| <I/σ(I)> | 63.9 | 8.2 |
| Completeness [%] | 99.5 | 96.4 |
| Redundancy | 11.7 | 10.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | Crystallized in 0.2M Na iodide, 20% PEG3350, 2% isopropanol, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
