2I7R
conserved domain protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-27 |
| Detector | SBC-3 |
| Wavelength(s) | 0.979321 |
| Spacegroup name | P 65 |
| Unit cell lengths | 97.624, 97.624, 55.151 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.560 - 2.200 |
| R-factor | 0.2 |
| Rwork | 0.198 |
| R-free | 0.25700 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.691 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.096 | 0.459 |
| Number of reflections | 15324 | |
| <I/σ(I)> | 8.5 | |
| Completeness [%] | 99.4 | 99.2 |
| Redundancy | 12.7 | 8.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 289 | 0.100 M sodium citrate 2.00 M sodium chloride, pH 4.50, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
