2I7G
Crystal Structure of Monooxygenase from Agrobacterium tumefaciens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-22 |
| Detector | SBC-3 |
| Wavelength(s) | 0.97908 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 99.515, 139.974, 54.049 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.370 - 1.730 |
| R-factor | 0.17292 |
| Rwork | 0.170 |
| R-free | 0.20199 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.405 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.370 | 1.790 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Rmerge | 0.086 | 0.503 |
| Number of reflections | 78621 | |
| <I/σ(I)> | 8.3 | 2 |
| Completeness [%] | 98.4 | 87.5 |
| Redundancy | 10.8 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295 | 0.1M Bis-tris pH 5.5, 0.2M Ammonium sulfate, 25% PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
