2I6S
Complement component C2a
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-18 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9393 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.160, 75.080, 71.560 |
| Unit cell angles | 90.00, 110.32, 90.00 |
Refinement procedure
| Resolution | 37.500 - 2.700 |
| Rwork | 0.212 |
| R-free | 0.28700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB Entries: 1DLE (SP-domain) and 1Q0P (VWA-domain) |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.670 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.540 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.085 | 0.420 |
| Total number of observations | 5710 | |
| Number of reflections | 13881 | |
| <I/σ(I)> | 7.4 | 1.8 |
| Completeness [%] | 98.5 | 90.4 |
| Redundancy | 3.6 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop vapor diffusion | 7 | 293 | 35% PEG 1000, 0.025 M sodium malonate, 0.0375 M imidazole, 0.0375 M boric acid, 20 mM LiCl, pH 7.0, hanging drop vapor diffusion, temperature 293K |
