2I5Z
The crystal structure of OspA mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-17 |
| Detector | SBC |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.783, 53.840, 65.495 |
| Unit cell angles | 90.00, 99.32, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.200 |
| R-factor | 0.14945 |
| Rwork | 0.148 |
| R-free | 0.17599 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2g8c |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.397 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.240 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.071 | 0.265 |
| Number of reflections | 70928 | |
| <I/σ(I)> | 15.59 | 2.43 |
| Completeness [%] | 95.2 | 91.6 |
| Redundancy | 2.3 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 34% PEG400, 0.1M Tris-HCl, pH 9, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
